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growth medium egm mv2  (PromoCell)


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    PromoCell growth medium egm mv2
    Growth Medium Egm Mv2, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egm+mv2/Endothelial+Cell+Growth+Medium+MV+2/pmc12647355-440-22-25
    Average 97 stars, based on 523 article reviews
    growth medium egm mv2 - by Bioz Stars, 2026-09
    97/100 stars

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    Cell Culture:

    Article Title: Microvasculature-on-a-Chip: Bridging the interstitial blood-lymph interface via mechanobiological stimuli
    Article Snippet: .. Microvascular cultures were maintained in EGM-2 or a mixture of EGM-MV (PromoCell) and EGM-MV2 (Promocell), depending on cultured cell types, with daily medium changes for five days. .. Primary lymphatic endothelial cell cultures were supplied with vascular endothelial growth factor C (VEGF-C, CoaChrom Diagnostica) at 25 ng/mL, 50 ng/mL, or 100 ng/mL and stimulated with 100 ng/mL Phorbol 12-Myristate 13-Acetate (PMA) as described.

    Article Title: Development of a functional airway-on-a-chip by 3D cell printing.
    Article Snippet: 1 We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel 2 network in vitro.. Decellularized extracellular matrix bioink derived from porcine tracheal 3 mucosa (tmdECM) was used to encapsulate and print endothelial cells and fibroblasts within 4 a designated polycarprolactone (PCL) frame.. Providing a niche that emulates conditions in 5 vivo, tmdECM gradually drives endothelial re-orientation, which leads to formation of a lumen 6 and a blood vessel network.

    Article Title: HIV Antivirals Affect Endothelial Activation and Endothelial-Platelet Crosstalk
    Article Snippet: Human umbilical cord vein endothelial cells (HUVEC) (PromoCell GmbH, Germany) were cultured in endothelial cell growth media (EGM)-2 (PromoCell GmbH) supplemented with 10% foetal calf serum (FCS). .. Human coronary artery endothelial cells (HCAEC) (PromoCell GmbH) were cultured in EGM-MV2 (PromoCell GmbH) supplemented with 10% FCS. .. Endothelial cells were cultured in 80cm2 NunclonTM Deltatreated tissue culture flasks (Thermo Scientific, UK) and seeded into 6-well NunclonTM Delta-treated tissue culture plates (Thermo Scientific) (50,000 cells/ml).

    Article Title: Endothelial cell–specific LAT1 ablation normalizes tumor vasculature
    Article Snippet: The 293FT cell line was purchased from Thermo Fisher Scientific. .. HUVEC were cultured in EGM-2 (PromoCell) and other microvascular endothelial cells were cultured in EGM-MV2 (PromoCell). .. For amino acid starvation, endothelial cells were cultured in custom-made amino acid deprived MCDB131 medium (Nacalai), which was supplemented by amino acids as needed according to the composition of MCDB131 media, containing 5 ng/mL VEGF (Peprotech), 50 ng/mL basic FGF (Peprotech), 8 U/mL heparin (Sigma-Aldrich), 2% dialyzed FBS (Thermo Fisher Scientific), and 5 U/mL penicillin/streptomycin (Nacalai).

    Article Title: Effect of the snake venom component crotamine on lymphatic endothelial cell responses and lymph transport.
    Article Snippet: Microcirculation.. 2022;00:e12775. wileyonlinelibrary.com/journal/micc | 1 of 11 https://doi.org/10.1111/micc.12775 © 2022 John Wiley & Sons Ltd. Abbreviations: IACUC, Institutional Animal Care and Use Committee; HDLECs, Human Dermal Lymphatic Endothelial Cells.. 1Department of Medical Physiology, Texas A&M University Health Science Center, Bryan, Texas, USA 2Center for Translational Cancer Research, Texas A&M Institute of Biosciences and Technology, Bryan, Texas, USA 3National Natural Toxins Research Center, Texas A&M Kingsville, Kingsville, Texas, USA Correspondence Walter Cromer, Department of Medical Physiology, Texas A&M University Health Science Center, Bryan, Texas, USA.

    Derivative Assay:

    Article Title: Effect of the snake venom component crotamine on lymphatic endothelial cell responses and lymph transport.
    Article Snippet: Microcirculation.. 2022;00:e12775. wileyonlinelibrary.com/journal/micc | 1 of 11 https://doi.org/10.1111/micc.12775 © 2022 John Wiley & Sons Ltd. Abbreviations: IACUC, Institutional Animal Care and Use Committee; HDLECs, Human Dermal Lymphatic Endothelial Cells.. 1Department of Medical Physiology, Texas A&M University Health Science Center, Bryan, Texas, USA 2Center for Translational Cancer Research, Texas A&M Institute of Biosciences and Technology, Bryan, Texas, USA 3National Natural Toxins Research Center, Texas A&M Kingsville, Kingsville, Texas, USA Correspondence Walter Cromer, Department of Medical Physiology, Texas A&M University Health Science Center, Bryan, Texas, USA.



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    (A) Control and IFT20 KD HDLECs in homeostasis were immunostained for IFT20 and RAB5. Micrographs are MIPs from laser scanning confocal microscopy. Scale bars = 25 μm. (B) Quantification of integrated intensity from RAB5+ area in control and IFT20 KD HDLECs. Intensities are graphed relative to average control HDLEC RAB5 intensity. Quantification is from one of three biological replicates and is representative of 300+ control and 300+ IFT20 KD HDLECs. (C) Control and IFT20 KD HDLECs were serum starved in media containing 1/5 <t>EGM-MV2</t> and 4/5 EBM-2 (hereafter, starving media) for 24 h. Cells were then either: placed in fresh starving media for 45 min, then fixed; placed in fresh starving media + 2 μg/mL VEGF-C for 45 min, then fixed; or placed in fresh starving media + 2 μg/mL VEGF-C for 45 min, then placed in EBM-2 basal media for a 90 min washout, then fixed. RAB5 was detected in fixed cells by immunofluorescence. Micrographs are MIPs from laser scanning confocal microscopy. Scale bar = 25 μm. (D) Quantification of C . Integrated intensities from RAB5+ area are graphed for control and IFT20 KD HDLECs separately and combined. Intensities are graphed relative to average control HDLEC RAB5 intensity in the 45 min starve condition. Data points represent the average of three FOVs within one technical replicate. Each of three independent experiments included two or three technical replicates (three FOVs each) per experimental condition. Quantification is representative of 300+ control and 300+ IFT20 KD HDLECs. *p<0.05, **p<0.005, ***p<0.001.
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    (A) Control and IFT20 KD HDLECs in homeostasis were immunostained for IFT20 and RAB5. Micrographs are MIPs from laser scanning confocal microscopy. Scale bars = 25 μm. (B) Quantification of integrated intensity from RAB5+ area in control and IFT20 KD HDLECs. Intensities are graphed relative to average control HDLEC RAB5 intensity. Quantification is from one of three biological replicates and is representative of 300+ control and 300+ IFT20 KD HDLECs. (C) Control and IFT20 KD HDLECs were serum starved in media containing 1/5 <t>EGM-MV2</t> and 4/5 EBM-2 (hereafter, starving media) for 24 h. Cells were then either: placed in fresh starving media for 45 min, then fixed; placed in fresh starving media + 2 μg/mL VEGF-C for 45 min, then fixed; or placed in fresh starving media + 2 μg/mL VEGF-C for 45 min, then placed in EBM-2 basal media for a 90 min washout, then fixed. RAB5 was detected in fixed cells by immunofluorescence. Micrographs are MIPs from laser scanning confocal microscopy. Scale bar = 25 μm. (D) Quantification of C . Integrated intensities from RAB5+ area are graphed for control and IFT20 KD HDLECs separately and combined. Intensities are graphed relative to average control HDLEC RAB5 intensity in the 45 min starve condition. Data points represent the average of three FOVs within one technical replicate. Each of three independent experiments included two or three technical replicates (three FOVs each) per experimental condition. Quantification is representative of 300+ control and 300+ IFT20 KD HDLECs. *p<0.05, **p<0.005, ***p<0.001.
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    Image Search Results


    (A) Control and IFT20 KD HDLECs in homeostasis were immunostained for IFT20 and RAB5. Micrographs are MIPs from laser scanning confocal microscopy. Scale bars = 25 μm. (B) Quantification of integrated intensity from RAB5+ area in control and IFT20 KD HDLECs. Intensities are graphed relative to average control HDLEC RAB5 intensity. Quantification is from one of three biological replicates and is representative of 300+ control and 300+ IFT20 KD HDLECs. (C) Control and IFT20 KD HDLECs were serum starved in media containing 1/5 EGM-MV2 and 4/5 EBM-2 (hereafter, starving media) for 24 h. Cells were then either: placed in fresh starving media for 45 min, then fixed; placed in fresh starving media + 2 μg/mL VEGF-C for 45 min, then fixed; or placed in fresh starving media + 2 μg/mL VEGF-C for 45 min, then placed in EBM-2 basal media for a 90 min washout, then fixed. RAB5 was detected in fixed cells by immunofluorescence. Micrographs are MIPs from laser scanning confocal microscopy. Scale bar = 25 μm. (D) Quantification of C . Integrated intensities from RAB5+ area are graphed for control and IFT20 KD HDLECs separately and combined. Intensities are graphed relative to average control HDLEC RAB5 intensity in the 45 min starve condition. Data points represent the average of three FOVs within one technical replicate. Each of three independent experiments included two or three technical replicates (three FOVs each) per experimental condition. Quantification is representative of 300+ control and 300+ IFT20 KD HDLECs. *p<0.05, **p<0.005, ***p<0.001.

    Journal: bioRxiv

    Article Title: IFT20 regulates lymphatic endothelial cell-cell junctions via endocytic trafficking of VE-cadherin

    doi: 10.1101/2025.01.15.631989

    Figure Lengend Snippet: (A) Control and IFT20 KD HDLECs in homeostasis were immunostained for IFT20 and RAB5. Micrographs are MIPs from laser scanning confocal microscopy. Scale bars = 25 μm. (B) Quantification of integrated intensity from RAB5+ area in control and IFT20 KD HDLECs. Intensities are graphed relative to average control HDLEC RAB5 intensity. Quantification is from one of three biological replicates and is representative of 300+ control and 300+ IFT20 KD HDLECs. (C) Control and IFT20 KD HDLECs were serum starved in media containing 1/5 EGM-MV2 and 4/5 EBM-2 (hereafter, starving media) for 24 h. Cells were then either: placed in fresh starving media for 45 min, then fixed; placed in fresh starving media + 2 μg/mL VEGF-C for 45 min, then fixed; or placed in fresh starving media + 2 μg/mL VEGF-C for 45 min, then placed in EBM-2 basal media for a 90 min washout, then fixed. RAB5 was detected in fixed cells by immunofluorescence. Micrographs are MIPs from laser scanning confocal microscopy. Scale bar = 25 μm. (D) Quantification of C . Integrated intensities from RAB5+ area are graphed for control and IFT20 KD HDLECs separately and combined. Intensities are graphed relative to average control HDLEC RAB5 intensity in the 45 min starve condition. Data points represent the average of three FOVs within one technical replicate. Each of three independent experiments included two or three technical replicates (three FOVs each) per experimental condition. Quantification is representative of 300+ control and 300+ IFT20 KD HDLECs. *p<0.05, **p<0.005, ***p<0.001.

    Article Snippet: HDLECs were routinely cultured in Endothelial Cell Growth Media MV2 (EGM-MV2) (PromoCell, C-39226) at 37°C and 5% CO 2 .

    Techniques: Control, Confocal Microscopy, Immunofluorescence